Culture Minister Ghulam Jamal said the film was blasphemous.
The screen adaptation of Dan Brown’s book revolves around the theory that Jesus married Mary Magdalene and their descendants survive today.
A number of states in India have banned the film, although the federal censors have cleared it for release.
Mr Jamal said: “Islam teaches us to respect all the prophets of Allah Almighty and degradation of any prophet is tantamount to defamation of the rest,” the official Associated Press of Pakistan news agency reported.
The government had indicated last week that it wanted to ban both the film and the book. The book has been available in Pakistan for some time.
Christian protestors have been supported by the country’s six-party Islamic alliance, the MMA.
On Saturday, Andhra Pradesh became the seventh state in India to ban the film.